The organophosphorus pesticides in soil was degradated by Rhodobacter sphaeroides after wastewater treatment

Abstract The bioremoval of organphosphorus and bioremovalof fertility in soil using Rhodobacter sphaeroides (R. sphaeroides) in the treatedwastewater were investigated in this research. Organphosphorus was not degraded under control group. The treated wastewater containning R. sphaeroides degraded organphosphorus and remediated fertility. Interestingly, the organophosphorus acid hydrolase gene ( opdA ) was expressed after inoculation 24 h. Subsequently, the organophosphorus acid hydrolase were synthesized to degrade organphosphorus. The organphosphorus started to be degraded after inoculation 24 h. The organphosphorus as stimulus signal induced organophosphorus acid hydrolase gene ( opdA ) expression through signal transduction pathway. The induction and secretion of the organophosphorus acid hydrolase occured after 24 h in R. sphaeroides , which could be attributed to its characteristics as an ancient bacteria. Lack of organic matter in soil under the control treatment could not maintain R. sphaeroides growth for over 24 h. The residual organics in the wastewater provided sufficient carbon sources and energy for R. sphaeroides under three dosage groups. The new method completed the remediation of organphosphorus pollution, the improvement of soil fertility and soybean processing wastewater treatment simultaneously, and realized the resource reutilization of wastewater and R. sphaeroides as sludge.