LC/API/MSによる薬物の高感度定量分析法のノウハウ

LC/API/MS enables quantitative analysis of drugs with high sensitivity. In order to obtain fine mass spectra, know-how for high sensitivity analysis is required. A stable spray can produce stable ionization for LC/MS analysis. It is also necessary to purify the samples for quantitation with high sensitivity. In addition to those, adequate analytical conditions of HPLC, LC/API/MS interface, and SIM (or SRM/MS) are important for analysis. Fragmentations observed in EI, CI, FAB, and LC/ESI mass spectrometry gave us hints for the selections of mass numbers for SRM analysis. LC/MS/MS analysis of enables quatitation of pg/ml level of drugs in plasma.