Регенерация глубоких резаных ран кожи при различных вариантах местной терапии

Background. Deep cut wounds are one of the most common injuries in accidents and still remain a global public health problem. Despite much success in treatment of wounds searching for and developing new medicines for local treatment are still relevant. The study objective was to test a possibility of local treatment with microbial transglutaminase (MTG) supplemented with an auxiliary experimental substance of the author’s herbal ointment by studying morphogenesis of skin regenerative processes on a model of full-thickness cut wounds. Methods. We studied the dynamics of skin regenerative processes using the following groups: no treatment; a standard treatment with the medicine Solcoseryl in combination with the author’s ointment; the ointment alone; and the ointment in combinations with the enzyme MTG at different concentrations. A set of morphological methods was used for studying the skin structure at organ, tissue, cell, and subcellular levels in experiments on 150 white male mice. The methods included macroscopic description and measurement of skin lesions; histological methods at the light-optical level for qualitative evaluation of skin morphologic changes; and transmission electron microscopy for identifying ultrastructural transformations of different epidermal and dermal cells. Results. Visual evaluation and histological study showed that he most effective treatment for healing of full-thickness cut wounds was 0.1% MTG as evidenced by arrest of bleeding and formation of a fibrin clot, which occurred three times faster than inother experimental and control groups; an early onset of epithelialization on day 3 of the experiment; and the completion of regenerative processes by day 10, which suggested prospective benefits of using transglutaminases in surgery and aesthetic medicine. Conclusions. The innovative local treatment of deep cut wounds of the skin with microbial transglutaminase supplemented with an auxiliary experimental substance of the author’s herbal ointment is characterized by an early onset of epithelization, which suggested prospective benefits of usingtransglutaminases in surgery and aesthetic medicine. At the same time, mechanisms of reparation need further investigation in clinical trials.