Additional file 3: of Distinct T-helper cell responses to Staphylococcus aureus bacteremia reflect immunologic comorbidities and correlate with mortality

Figure S2. Method used to classify T cells and CD3+CD4+ cells. CD4+ T cells were first separated into CD25+CD127− (Tregs) or conventional T cells (Tconv). The Tconv were further characterized by expression of CD45RO as a marker of effector/memory T cells. The CD45RO+ cells were then divided into Th1, Th2, or Th17 cells based on the expression of CCR4 and CCR6, with Th1 identified as CCR4−CCR6−, Th2 identified as CCR4+CCR6−, and Th17 identified as CCR6+. (PPTX 84 kb)