Genetic analyses of archival specimens of the Atlantic sturgeon Acipenser sturio L., 1758

Genetic variability was analysed in Acipenser sturio L., 1758 and Acipenser oxyrinchus oxyrinchus Mitchill, 1815 using variation in the D-loop region of mtDNA and a number of microsatellites (nuclear markers). The studied material included tissue samples from: (1) 38 A. sturio archival specimens collected in different German, Swedish, Danish, and French museums of natural history; (2) 27 live A. sturio representing a broodstock for restoration of this species in German waters (Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany); (3) 30 wild A. o. oxyrinchus caught in the Atlantic Ocean near the coast of New Jersey and in the Delaware River (USA); and (4) 60 individuals of A. o. oxyrinchus from an artificially reproduced stock originally obtained from several wild sturgeons captured in the St. John River (Canada). A 250-bp fragment of the D-loop region of mtDNA was cloned and sequenced. The length of a repeated unit was 80 bp in A. sturio and 79 bp in A. o. oxyrinchus. The repeated units of A. sturio and A. o. oxyrinchus differed by 11 substitutions and one deletion or insertion, respectively. No heteroplasmy was found. Three different haplotypes of mtDNA were observed in both species. Five microsatellites had polymorphic band patterns. In A. sturio, analyses of microsatellites showed a decrease in allelic numbers between the years 1823 and 1992. This decline resulted in a fixation of several alleles. For A. sturio, one mtDNA haplotype and seven alleles were observed only in archival samples. Genetic distance calculations showed a great genetic similarity between A. sturio populations in the Gironde River and the North Sea, and a basal position of the Mediterranean and Adriatic Sea A. sturio populations. In A. o. oxyrinchus, the number of microsatellite alleles ranged between 14 (Hudson and St. John rivers) and 22 (Delaware River). Genetic distance calculations showed a high genetic similarity between subpopulations of A. o. oxyrinchus.