Effects of Carnoy' s Solution and TCA on the Pouch Mucosa of Hamster

Objective. To inve-<;tigatethe injury of Carnoy' s solution and trichloracetic acid (TCA) on the pouch mu- cosa of hamster, and provide evidence for these agents to be used in keratocyst treatment. Methods. 20 hamsters were employed as research objects, and divided into 4 groups randomly; Camoy's group, 20% TCA group, 35% TCA group, and control group. The cheek pouches of hamster were treated with freshly prepared Carnoy' s solu- tion, 20% TCA and 35% TCA separately for 3 minutes. The specimens were taken immediately, and at 1,3,6, 12 weeks after operation. HE staining method, Van Gieson staining method, enzymohistochemistry and Transmis- sion electron microscopy were used to study the effects of the-<;e agents on the mucosa. Results. Both Carnoy' s solu- tion and 35 % TCA caused formation of fistula on pouch at the first week. Microscopic observation showed the ep- ithelia formed an unorganized necrosis rone, and cells lose their morphous in Carnoy' s solution group. The penetra- tion depth was about 0.3 mm. In both 20 % and 35 % TCA group, the epithelia cell showed nuclear fragmentation or delique-<;cence, and internal structure lost, leading to the cell a vacuolar shadow. Arrangement of collagen fibers under epithelia was disordered; and fibroblast was disappearing in all te-<;tgroups in the first week. From the 3rd week to the 6th week, the collagen fibers and fibroblast in all te-<;tgroups were hyperplasia. Conclusion. Carnoy's solution and TCA have great penetrating power on the mucosa of cheek pouch, and they can destroy the epithelia and subcutaneous tissue-<;. If these solutions could be for keratocyst therapy, they might cure the keratocyst or de- crease recurrence rate. (Life Science Journal. 2006; 3(2); 35 - 40) (ISSN; 1097 - 8135).