Fourier Interferometry as Applied to Microspectrofluorometry and Fluorescence Imaging of Living Cells

Excitation-emission fluorescence spectroscopy and imaging are applied to studies of cellular metabolism and at the convergence of cellular differentiation, detoxification, transformation, and senescence. Metabolic activity, intracellular redox levels, and compartmentation are probed by coenzyme [NAD(P)H] transients and monochlorobimane for glutathione dehydrogenase. Gene expression or its failure in lysosomal disorders is identified with fluorogenic probes. The "multiorganelle detoxification complex" is visualized and investigated with cytotoxic agents. A kind of photoactivated "accelerated cellular senescence" is recognized by accumulation of Schiff bases. Conventional and novel mitochondrial probes are used to localize these organelles in Saccharomyces cerevisiae as a model for future studies in mammalian cells and to detect in these very cells the organelle interactions resulting from the action of mitochondria-toxic drugs. The potential of these studies for biotechnology and instrumentation relying on...