Evaluation of glycogen level in human lung carcinoma tissues by an infrared spectroscopic method

Abstract Glycogen levels in the tissue samples obtained from carcinomas and normal sections of human lungs (26 patients) were studied by measuring the infrared band intensity at 1045 cm −1 due to glycogen. As an internal standard peak, the band at 1545 cm −1 (amide II) was chosen, and the ratios of these band areas ( A 1045 A 1545 ) were compared with histological classification and differentiation of tumors. The glycogen level in the carcinoma tissues was significantly higher than that in the normal tissues ( P n = 26). Further, the ratio of amounts of glycogen in the carcinomas and in the normal tissues for adenocarcinoma was higher than that for squamous cell carcinoma ( P