S77. Proffered paper: In vitro induced response patterns of antileukemic T-cells – characterization by combination of functional assays, spectratyping and next generation sequencing

Background T-cell receptor (TCR) diversity is characterised by somatic alterations in the complementary determining region 3 (CDR3) of the human TCR b-chain. Complemented with the TCR alpha-chain, TCR diversity can hypothetically result in up to 10 different TCR molecules. Myeloid leukaemic cells can be induced to differentiate into leukaemia-derived dendritic cells (DCleu) regaining the stimulatory capacity of professional DCs while presenting the whole leukaemic antigen repertoire. Our aim was to identify TCR Vb-chain-rearrangements in T-cells stimulated with leukaemic blasts and DCleu in 3 patients with AML and furthermore to detect, amplify or monitor T-cell clones with defined Vb-profiles in correlation with antileukaemic function, in vitro and in vivo.