Antisense Ki-67 cDNA Transfection Reverses the Tumorigenicity and Induces Apoptosis in Human Breast Cancer Cells

Effects of antisense Ki-67 cDNA transfection on breast cancer cells were investigated in this study. Transfection of antisense Ki-67 cDNA resulted in a 70%–80% reduction in proliferation of MDA-MB-435s, cells which highly expressed Ki-67 mRNA and protein. Transwell assay showed that mobility and invasion capability was dramatically inhibited by 50%–60%, and cell cycle analysis displayed a higher proportion in G2/M and G0/G1 phases accompanied by remarkably increased ratio of apoptotic cells. Our results suggested that antisense Ki-67 cDNA vector treatment might be an important potential option in the anticancer therapy.