Expression of the Escherichia coli Ada Regulon in Stationary Phase: Evidence for rpoS-Dependent Negative Regulation of alkA Transcription

The Escherichia coli Ada protein activates ς70-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA. During stationary phase, however, the methylated form of Ada shuts off expression of alkA; this repression is specific for ςS-dependent transcription. Thus, at the alkA promoter, the Ada protein can act as both a positive and negative modulator of the adaptive response to alkylation damage, depending on the cell’s physiological state.