Isolation and bioactivity of endophytic fungi in Derris hancei.

Sixteen endophytic fungi belonging 10 genus were isolated from Derris hancei Hemsl.The antagonism of endophytic fungi against fungal pathogens was tested in vitro.Penicillium sp.Q1,Rhizoctonia sp.S1,Phomopsis sp.N2,and Corticium sp.F1 isolated from the caudex of D.hancei,and Penicillium sp.Q2 isolated from the leaf,inhibited the hyphal growth of Colletotrichum gloeosporioides Penz,Fusarium oxysporum f.niveum(E.F.Smith)Snyber et Hansen,Rhizoctonia sp.S1 against Colletotrichum orbiculare Arx,and Phomopsis sp.N2 against Colletotrichum musae(Berk1 Curt1) Arx1 on dual culture with inhibition index II.It was reported that endophytic fungus in D.hancei could produced antibacterial substances in this paper.The culture filtrates of Penicillium sp.Q2 treated in 48 h after treatment possessed 100.00% of adjusted mortality against the 2nd larvae of Spodoptera litura by leaves disc feeding bioassays,and 75.10% against Lipaphis erysimi Kaltenbach(apterous adult) by insect-soaking method,respectively,which showed that the activity of Penicillium sp.Q2 was higher than that of other endophytic fungi.