A metabolomic study on the association of exposure to heavy metals in the first trimester with primary tooth eruption

Abstract Background The influence of prenatal heavy metals exposure on primary tooth eruption in humans is rarely reported. Aim Based on the cohort study design, we investigated the association of exposure to 12 heavy metals in the first trimester with primary tooth eruption, and the maternal metabolisms in the first trimester which might be related to the above relationship. Methods Maternal urine samples were collected in their first trimester, and 12 metals (Ti, V, Fe, Co, Cu, As, Se, Cd, Sn, Hg, Tl, U) were measured using the inductively coupled plasma mass spectrometry method. The maternal metabolome in the first trimester was analyzed by ultrahigh performance liquid chromatography coupled mass spectrometry based metabolomics using urine samples. The infant's first tooth eruption time and number of teeth at age one were recorded by oral examination and questionnaire. Results No significant associations were observed between heavy metals exposure in the first trimester and primary tooth eruption, except for Co. The level of Co was positively associated with time of infant's first tooth eruption, and was negatively associated with the number of teeth at age one. Based on metabolomic profiling, glycine was revealed as the key mediating metabolite, which showed negative correlation with Co and opposite effect of Co in the primary tooth eruption. Conclusions Prenatal Co exposure in the first trimester might delay the primary tooth eruption in children through the decreased glycine-disrupted dentin formation, providing the first evidence and novel insights into the control of prenatal heavy metals exposure for ensuring normal (timely) primary tooth eruption.