Subtyping of factor B by agarose gel electrophoresis

Factor B (BF) polymorphism has been extended by the occurrence of subtypes in each of two common alleles, BF*F (Teng and Tan 1982; Geserick 1983; Abbal 1985; Nagai 1986; Nakamura 1987; Nishimukai 1987) and BF*S (David 1983; Weidinger 1984). Isoelectric focusing (IEF) has contributed to these studies, but there is a lack of correspondence among the subtypes postulated by each group or their frequencies. We present a BF F subtype using agarose gel electrophoresis (AGE) in Tris/glycine/Veronal buffer, further characterize them by zymosan treatment and by IEF in polyacrylamide gels (PAGIF) and also show a BF S subtype in the Hungarian population.