INVESTIGATING EFFICIENCY OF PCR METHOD IN DIAGNOSIS OF BRUCELLOSIS, COMPARING TO SEROLOGIC METHODS

Brucellosis is one of the common diseases between human and animals that has worldwide outbreak. The mentioned disease is important due to its economic and health effects. Common diagnosis methods of brucellosis are always faced many limitations and problems in the laboratories. Hence, currently researchers have focused their attention on the molecular methods, especially PCR method. At the present study, sensitivity and efficiency of PCR method in diagnosis of brucellosis, comparing to serologic methods, has been evaluated. For this purpose, 30 blood samples suspected to brucellosis, along with their culture results, have been collected from the department of infectious diseases of Imam Khomeini Hospital Tehran. Then, common tests of serology such as Tube Wright, Rapid Wright, Rose Bengal, and 2ME tests have been conducted on all blood samples. DNA has been then extracted from all provided samples and then PCR test has been conducted using specific primers of genus. Perfect sensitivity of PCR method at the present study has proved high efficiency of the mentioned test in correct diagnosis of patients, especially endangered individuals. KAYWORDS: Brucellosis,PCRMethod,SerologicMethods,DNAExt raction