Chemical constituents of Penicillium sp. MA-37,a fungus derived from rhizospheres of mangrove plant Bruguiera gymnorrhiza

From the fermentation culture of strain Penicillium sp.MA-37 derived from mangrove rhizosphere,eight compounds were isolated by separation techniques including column chromatography with silica gel and Sephadex LH-20,preparative TLC and re-crystallization.Structures of all purified compounds were elucidated by analysis of their spectroscopic data to be N-(2-methoxy-4-hydroxyphenyl)-malonamic acid(1),N-(2,4-dimethoxyphenyl)malonamic acid(2),N-(2,4-dimethoxyphenyl)-malonamic acid methyl ester(3),(22E,24R)-ergosta-5,7,22-trien3β-ol(4),(22E,24R)-Ergosta-4,6,8(14),22-tetraen-3-one(5),(22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(6),asperamide B(7),cis-4-hydroxy-6-deoxyscytalone(8).Compound 1 was a new compound,while compounds 2 and 3 were reported as natural products for the first time.