A stereotaxic method of recording from single neurons in the intact in vivo eye of the cat.

Abstract A method is described for recording stereotaxically from single retinal neurons in the optically intact in vivo eye of the cat. The method is implemented with the help of a new type of stereotaxic instrument and a specially developed stereotaxic atlas of the cat's eye and retina. The instrument is extremely stable and facilitates intracellular recording from retinal neurons. The microelectrode can be rotated about two mutually perpendicular axes, which intersect in the freely positionable pivot point of the electrode manipulation system. When the pivot point is made to coincide with a small electrode-entrance hole in the sclera of the eye, a large retinal region can be reached through this fixed hole in the immobilized eye. The stereotaxic method makes it possible to choose a target point on the presented eye atlas and predict the settings of the instrument necessary to reach this target. The method also includes the prediction of the corresponding light stimulus position on a tangent screen and the calculation of the projection of the recording electrode on this screen. The sources of error in the method were studied experimentally and a numerical perturbation analysis was carried out to study the influence of each of the sources of error on the final result. The overall accuracy of the method is of the order of 5° of visual angle, which will be be sufficient for most purposes.