Calorie restricted diet induces alternative pathways of lipid metabolism for support of proliferative processes in regenerating liver.

: This study deals with quality and quantity lipid composition of blood serum and liver, DNA synthesis activity (incorporation of H3-thymidine) in liver in 24 h after partial hepatectomy (PH) in 22-month-old Wistar rats, maintained for 21 months on calorie restricted diet (CRD) and on standard diet ad libitum (SD). The contain of lipids in blood serum and activity of RA-label incorporation (14C-Na-acetate) in serum lipids in 24 h after PH were the same in CRD-fed and SD-fed animals. Quantitative and qualitative composition of lipids in microsomes of regenerating liver also was the same for both groups of rats. In regenerating liver of CRD-fed animals lipid contain in cytosol was 1,8-fold more, but pool of lipid droplets (LD) was 1.5-fold less than in regenerating liver of SD-fed animals. Activity of RA-label incorporation in lipids of microsomes, cytosol and LD pool of regenerating liver of CRD-fed animals was significantly higher, than in SD-fed ones. Activity of RA-label incorporation in lipid fractions and its distribution among cytosol lipids and LD pool lipids differed significantly between SD- and CRD-fed animals. Activity of DNA synthesis in regenerating liver of 22-month-old animals on CRD and SD was the same. It is supposed that calorie restriction induces alternative pathways of lipid metabolism to support proliferation processes in liver after PH.