Production of peptides from the tilapia skin proteins by fermentation

Objective:The production technology of producing peptides from the tilapia skin proteins by fermentation with Bacillus subtilis was studied.Method:Taking the tilapia skin and water an substrate,Bacillus subtilis was added as ferment.The tilapia skin concentration,liquid volume,cell-age,inoculum size and incubation time were observed.the optimal fermentation conditions were optimized by single factor and orthogonal tests.Result:the optimal fermentation conditions were as follow:the tilapia skin concentration 3%,liquid volume 70 mL/250 mL,cell-age 14 h,3% inoculum size,incubation time 52 h,and under the above fermentation conditions the degree of hydrolysis of the tilapia skin proteins could reach 36.88%.Conclusion:The method of producing peptides from the tilapia skin proteins by fermentation with Bacillus subtilis has many advantages such as simple technology,low cost,no polluting environment and high degree of hydrolysis,which should be to promoted to produce the collagen peptides.