Unusual conformation of a 3′-thioformacetal linkage in a DNA duplex

1994 
The DNA·DNA duplex \({\text{d}}\left( {{\text{CGCGTT}}_{{\text{SCH}}_{\text{2}} {\text{O}}} {\text{TTGCGC}}} \right)\)·d(GCGCAAAACGCG) (designated duplex III) containing a 3′-thioformacetal (3′-TFMA) linkage in the center of the sequence was characterized in detail by two- and three-dimensional homonuclear NMR spectroscopy. The NMR results were analyzed and compared with those of two duplexes of the same sequence: One is an unmodified reference sequence and the other contains a formacetal (OCH2O) linkage at the central T^T step (designated duplex I and duplex II, respectively). In general, the NMR spectra of duplex III closely resemble those of the analogous duplexes I and II, suggesting an overall B-type structure adopted by the 3′-TFMA-modified duplex III. Nonetheless, the detection of several distinct spectral features originating from the protons at the \({\text{T6}}_{{\text{3' - SCH}}_{\text{2}} {\text{O}}} {\text{T7}}\) modification site is indicative of a local conformation that is clearly different from the corresponding region in duplexes I and II. The 3′-thioformacetal linker, in contrast to the formacetal (FMA) linkage, cannot be accommodated in a conformation usually found in natural nucleic acid duplexes. As a consequence, the 3′-TFMA-modified T6 sugar adopts an O4′-endo form (an intermediate structure between the usual C2′-endo and C3′-endo forms). This change is accompanied by a change in the e (C4′−C3′−S3′−CH2) dihedral angle and by subsequent adjustments of other torsion angles along the backbone. Notably, this conformational readjustment at the T6–T7 backbone linkage is localized; its collective result has negligible effect on base-base stacking of the T6 and T7 residues. A close examination of the COSY data in all three duplexes reveals a subtle variation in sugar geometry, with more S-type character adopted by the modified duplexes II and III. The results of this study illustrate that, although the difference between FMA and 3′-TFMA linkages is merely in the substitution of the T6(O3′) in the former by a sulfur atom in the latter, the stereoelectronic difference in a single atom can induce significant local structural distortion in an otherwise well-structured oligonucleotide duplex.
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