Establishment of a PCR-LDR genotyping system for population construction of chromosome 1 substitution strains from wild mice

Objective To establish a PCR-LDR genotyping system for the population construction of chromosome 1 substitution strains from wild mice.Methods The single nuclear polymorphisms(SNPs) were genotyped based on the polymerase chain reaction and ligase detection reaction(PCR-LDR).Results based on PCR-LDR,a total of 29 SNPs with the average density of 6.25 centimorgan(cM) were designed.All the SNPs were included in three panels,and each panel contained at least 9 SNPs.Conclusion The PCR-LDR genotyping system can be used to accurately detect the recombination events,with convenience and high throughout.