405 Clinicopathological features associated with immunohistochemistry loss of mismatch repair protein mlh1 in endometrioid endometrial carcinoma

Objectives The aim of this study was to assess the association between DNA mismatch repair protein MLH1 status and classical prognostic factors in patients with endometrioid endometrial carcinoma. Methods We studied 47 consecutive patients aged 37–88 (61.34±10.52) with confirmed diagnosis of endometrial carcinoma of endometrioid type. The tumors were classified according the WHO 2014 criteria and graded using the FIGO system. The following pathological characteristics were analyzed: tumor size (mm), percentage of myometrial infiltration, presence of microcystic, elongated, and fragmented (MELF) pattern of myoinvasion, percentage of tumoral infiltrating lymphocytes (TILs), and lymph-vascular space invasion (LVSI). Deficiency of MLH1 protein was defined as complete loss of nuclear expression within tumor cells in the presence of positive internal controls in lymphocytes and/or stroma. The chi-square test and the Mann-Whitney U test were used to compare the groups with and without MLH1 loss. Results Loss of MLH1 was observed in 18/47 (38.3%) patients. Age of patients did not differ between MLH1-negative and MLH1-positive tumors (61.2±11.0 years and 61.4±10.4 years, respectively). The comparison of characteristics of the tumors with and without MLH1 loss is summarized in table 1. Conclusions Loss of MLH1 was associated with bigger tumors, MELF pattern of myoinvasion, and positive lymph nodes. Tumors with MLH1 loss presented more myometrial infiltration, more LVSI, and more TILs, although it did not reach a statistical level of significance. The presence of higher percentage of TILs, as well MLH1 loss itself, may indicate immunotherapy susceptibility.