DNA amplification on a PDMS?glass hybrid microchip
2006
In this paper, the development of a hybrid polydimethylsiloxane (PDMS)?glass microchip for PCR (polymerase chain reaction) was reported. A novel temperature control strategy was applied for precise temperature control. The microchip was composed of two parts?a PDMS substrate and a glass cover. The PDMS substrate includes a reaction chamber for PCR and two microchannels for the inlet and the outlet of the sample. A glass cover integrating heaters and sensors was bonded to the PDMS substrate. The microchip was produced by molding PDMS against a microfabricated master with comparatively simple and inexpensive methods. PCR was successfully carried out on the PDMS?glass hybrid microchip with a 183 bp target of DNA.
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