Development and Validation of RP-HPLC Method for Simultaneous Estimation of Oxyclozanide and Tetramisole Hydrochloride from Formulation

A simple, precise, accurate, and rapid high performance liquid chromatography (HPLC) method has been developed and validated for the determination of oxyclozanide and tetramisole hydrochloride simultaneously, in combined pharmaceutical solid dosage form. The mobile phase used was mixture of solution (2 ml TEA in 1 litre milli-Q water, pH adjusted to 3 with OPA) and acetonitrile (30:70 v/v). Flow rate was set to 1 ml/min. The detection of Oxyclozanide and Tetramisole Hydrochloride was carried out on absorbance detector at 215 nm. Results of the analysis were validated statistically.