Ac/Ds transposon system and its application strategy on construction of rape mutants pool

With the completion of the rape(Brassica campestris)genome sequence and its subsequent improvement,the study on functional genomics in rape has come into focus,therefore,how to construct high throughput mutants pool is the key affecting its advances.Ac/Ds(Activator/Dissociation)system is a promising method to construct insertional mutants pool of heterologous plant such as Oryza sativa and Arabidopsis thaliana,however it rarely has information related to rape.In this paper,difficulties and advantages of using Ac/Ds system,characteristics and mechanism of Ac/Ds system were reviewed.Combined with the current achievements on rice and Arabidopsis thaliana,the improvement strategies for construction of rape mutants pool by Ac/Ds system were analyzed further,and the application prospects of Ac/Ds system used in rape functional genomics were summarized.