Antigenic homologies between oat and wheat globulins

Abstract Total globulins of oat and wheat were compared by SDS-electophoresis immunoblotting and in vitro protein synthesis experiments. Numerous homologies were found. The same 4 protein subgroups, with respect to molecular mass, were found in the two cereals. Immunoblotting with antibodies for total oat globulins allowed the detection of homologous proteins in each wheat protein group, especially in the 60-kDa group, which corresponds to the major 12 S oat globulin. The same antibodies also reacted with in vitro synthesized proteins of the two cereals. However, compared to mature proteins, only a limited number of polypeptides react, suggesting that wheat globulins, like oat globulins, undergo post-translational processes.