Isolation of a Yeast Artificial Chromosome Clone That Spans the (12;16) Translocation Breakpoint Characteristic of Myxoid Liposarcoma

Cytogenetic analysis of liposarcomas has demonstrated that translocation (12;16) (q 13.3 ;pl 1.2) is characteristic of the myxoid subtype of this adipose tissue tumor. Our previous results suggested that the GLI gene is close to the translocation breakpoint on chromosome 12. We now describe a yeast artificial chromosome (YAC) that contains GLI and spans the chromosome 12 region involved in the t(12;16) breakpoint. This clone will permit rapid definition of the genetic region surrounding the breakpoint and allow isolation of the gene presumably affected by the translocation.