Application of Polyamide Resin in Purification of Hyperoside from Hypericum perforatum L.

Objective:To study the technological parameters of the purification process of hyperoside from Hypericum perforatum extrative.Methods: HPLC was used to analyze the content of hyperoside in the samples from the different technological process,and the adsorption and elution parameters were investigated.Results: The optimal parameters were as follows:the sample extract solution,whose content of hyperoside was 1.2 mg·mL~(-1),was passed through the packed column with a flow rate of 3.6 BV·h~(-1).The adsorption rate of hyperoside was 2.33 mg·g~(1),and the purity of hyperoside in the eluate reached 28.96%.Conclusion: Polyamide resin can be used to purify hyperoside in the extraction of Hypericum perforatum L.