RNA editing analysis of Phosphoprotein gene of velogenic Newcastle disease virus from goose and molecular characteristic analysis of its encoded proteins.

Transcriptional editing of Phosphoprotein(P) gene of Newcastle disease virus of WF00G strain from Goose was analysed by reverse transcription polymerase chain reaction(RT-PCR).The results showed that one G nucleotide addition was detected at the conserved transcriptional editing site that would allow for a potential V protein encoded by P gene mRNA,and the most open reading frame(ORF) of P gene contained 1188 bp and encoded phosphoprotein with 395 amino acids.Sequence alignment and phylogenetic analysis of P genes of WF00G strain and reference strains demonstrated that P gene of WF00G strain was more homologous to virulent strains from waterfowl(NA-1,ZJ1,FP1/02,PX2/03,Duck/1/05 and SF02) than to vaccine strains or low virulent strains(HB92,LaSota,Clone30 and F48E9).Comparision of the carboxyl terminus amino acid sequences of V protein of WF00G strain and reference strains revealed that the amino acid sequences of editing site(132KKG134)and five cysteine residues at the carboxyl terminus of V protein were highly conservative among all strains,but many variations exist at the carboxyl terminus of V protein.The mutation of V protein at carboxyl terminus among all strains showed "regularity of genotype".