Mesenchymal stem cells derived from rat bone marrow (rbm msc): techniques for isolation, expansion and differentiation

Mesenchymal stem cells derived from bone marrow have significant capacity for attachment expansion and multiline age differentiation This property has been widely used in the clinical practice for treatment of variety of diseases Stem cell collection and culturing techniques varies for different species Time taken for the primary cell culture to attain confluency depends on the species from which bone marrow is isolated This paper describes an easy technique for the isolation and differentiation of mesenchymal stem cells derived from rat bone marrow r MSCs on the basis of their density gradients and plastic adherence property along with the multiline age differentiation potential After isolation characterization was performed morphologically by examination under phase contrast microscope and viability detected by cell viability assay Spindle shaped morphology was exhibited by the attached nucleated cells Their multiline age differentiation to osteoblast adiposities osteoblast and chondroblasts was examined with respective induction medium and confirmed by oil red O alizarin red and Alcian blue stains respectively The r MSCs were cultured and passaged for their expansion and were maintained in suitable environment for long time and can be used for cell based regenerative medicine