Development of high sensitivity immunochromatographic test card for zearalenone

OBJECTIVE: To develop a highly sensitive immunochromatographic test card for zearalenone. METHODS: Gold nanoflowers(AuNFs) with a particle size of(116. 7±2. 0) nm and multiple branches was prepared and labeled to the zearalenone monoclonal antibody. Based on the gold nanoflower-labeled zearalenone monoclonal antibody(AuNF-ZEN-Mab), a zearalenone gold nanoflower immunochromatographic test card(ZEN-GFICT) was developed for the detection of food and feed. RESULTS: The detection sensitivities of ZEN-GFICT(contrast method) and(cancellation method) for ZEN standards were 0. 5 and 6 ng/mL, respectively, and the limit of detection(LOD) for actual samples were 5 and 60 μg/kg, respectively. It was 6 times(contrast method) and 4. 5 times(cancellation method) of the traditional ZEN nano gold immunochromatographic test card(ZEN-GICT). The total conformity of the ZEN-GFICT judgment result(contrast method and cancellation method) of 21 samples compared with those of the ELISA kit were 90. 5% and 90. 5%, respectively. CONCLUSION: The ZEN content in the sample with three intervals of less than 5 μg/kg, 5-60 μg/kg and more than 60 μg/kg could be screened by the ZEN-GFICT contrast method and the cancellation method. It is possible to screen samples with a low degree of ZEN contamination.