Construction of Recombinant Duck Plague Virus with VP1 Gene of Duck Hepatitis Virus Type I

A study on safety of tulathromycin in swine was investigated to provide an experiment support for the clinical use of this drug.Eighteen pigs were randomly allocated to three treatment groups.A further six pigs were left untreated as controls(group NTXL).Tulathromycin was administered twice by the intramuscular(i.m.) route administrations of 2.5,7.5,12.5 mg/kg in three treatment groups on the first day and the fourth day,respectively.Blood samples were taken from all animals on days-1,1,3,6,10,15 for serum chemical and hematology evaluation,mass and temperature were measured from all animals at the same time.The injection sites became red in the group that was administered twice of 12.5 mg/kg,however,disappeared later.Other pigs did not show any signs of discomfort after injection.Hematology evaluation indicated no significant differences(P0.05) between treatment and control groups.Aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP) in serum,albumin(ALB)were significantly higher in treated groups when compared with the control group(P0.05) post-treatment.No signifcant differences was found with the increase of time interval.The safety of tulathromycin injection in target animal swine indicated that pigs were administered of 2.5-12.5 mg/kg by i.m.route and satisfied veterinary clinical demands.