TrypLE ™ Express: A Temperature Stable Replacement for Animal Trypsin in Cell Dissociation Applications

TrypLE ™ Express is a recombinant fungal trypsin-like protease, which has proven effective at dissociating many different attachment dependent mammalian cell lines. It has similar dissociation kinetics to porcine trypsin and exhibits lower cell toxicity. Cell replating, proliferation kinetics, and long-term maintenance were comparable to cells harvested using animal trypsin. HPLC analysis indicates a single peak corresponding to the active form of the recombinant enzyme, as opposed to the multiple peaks seen with animal (porcine pancreatic) trypsin. Studies of TrypLE ™ Express in solution have demonstrated remarkable enzyme stability. Enzyme assay results indicate that at 4˚C and room temperature, the enzyme remains stable for more than 12 months. Even at 37˚C, TrypLE ™ Express maintains 85% activity for 8 days. In contrast, porcine trypsin lost greater than 95% of its trypsin activity after just 24 hours at 37˚C. This temperature stability will allow more convenient storage and handling of this cell dissociation enzyme during routine cell culture procedures.