Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea
2018
A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T, was isolated from forest soil. Growth occurred at 10–40 °C (optimum 28–30 °C), at pH 6–8 (optimum 7) and at 0–4 % NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as
Actinotalea ferrariae
KCTC 29134T (97.9 %),
Actinotalea fermentans
KCTC 3251T (97.3 %),
Cellulomonas carbonis
KCTC 19824T (97.2 %). 16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid. The isoprenoid quinone was menaquinone (MK-10(H4)). The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The whole-cell sugars of strain THG-T121T were rhamnose, ribose, mannose and glucose. The peptidoglycan type of strain THG-T121T is A4β, containing l-Orn–D-Ser–L-Asp. The DNA G+C content of strain THG-T121T was 72.4 mol%. DNA–DNA hybridization values between strain THG-T121T and
A. ferrariae
KCTC 29134T,
A. fermentans
KCTC 3251T and C. carbonis KCTC 19824T were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T121T represents a novel species of the genus
Actinotalea
, for which the name
Actinotalea
solisilvae sp. nov. is proposed. The type strain is THG-T121T (=KACC 19191T=CGMCC 4.7389T).
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