Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme

Venkata B. Nanduri,Steven L. Goldberg,Robert J. Johnston,Ramesh N. Patel

Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme

2004

Venkata B. Nanduri
Steven L. Goldberg
Robert J. Johnston
Ramesh N. Patel

A novel enantioselective N-carbobenzyloxy (Cbz)-cleaving enzyme was purified to homogeneity from Sphingomonas paucimobilis SC 16113. The gene encoding the protein has been identified and its complete DNA sequence was determined. The Cbz-cleaving enzyme was expressed in Escherichia coli.

Keywords:

Enzyme
DNA sequencing
Enantioselective synthesis
Gene expression
Biochemistry
Sphingomonas paucimobilis
Escherichia coli
Molecular cloning
Gene
Biology
Molecular biology
Cloning
Chemistry

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