Method for extracting high-purity daptomycin by utilizing chromatographic technique

The invention discloses a method for extracting high-purity daptomycin by utilizing a chromatographic technique. The method comprises the following steps that firstly, pre-processing is carried out: filtering is carried out on fermentation liquor containing with the daptomycin, and the turbidity of the filtered daptomycin fermentation liquor is lower than a pre-set value; secondly, extracting is carried out: the fermentation liquor which is subjected to the processing in the first step is injected into a chromatographic column filled with first filter to be subjected to absorbing, the first filter is anion exchanging gel filter, wherein glucan or agarose serves as the framework of the anion exchanging gel filter, after the absorbing is carried out, eluent is added, and the daptomycin is eluted; thirdly, refining is carried out; obtained eluting liquid which is obtained in the second step is injected into a chromatographic column filled with second filter to be subjected to the absorbing, polystyrene-divinyl benzene or bonded silica gel serves as the framework of the second filter, after the absorbing is carried out, buffer salt eluting solvent is added, eluting is carried out, and therefore the high-purity daptomycin is obtained. According to the method, the technology is simple, the operating cost is low, and a final daptomycin product with the purity above 98% can be obtained.