Purified 14C Virginiamycin: Separation, Purification, and Recombination of 14C Factors S and M

Abstract 14C virginiamycin, an antibiotic composed of two synergistic factors S and M, was required for metabolism and residue studies. 14C Factors S and M were separated from crude 14C virginiamycin and purified by semi-preparative normal phase HPLC followed by crystallization/precipitation. After chemical and radiopurity assays, the two factors were recombined in a 1:4 ratio to form purified 14C virginiamycin.