Establishment of human bladder-cancer cell-line (hamt-1) and long-term subculture in nude-mice.

: Human bladder cancer cell line (HAMT-1) was established from a transitional cell carcinoma of the bladder (T4N3M0, grade 3) of a 61-year-old male by using a serum-free medium. HAMT-1 cells in serum-free medium were non-adhesive to the flask wall or to other cells. The cells grew in attached form by the addition of fetal calf serum. Heterotransplantation of HAMT-1 cells into nude mice and the long-term subculture were successful. Tumor bearing nude mice showed an elevated serum tissue polypeptide antigen as in the patient.