Analysis different methods between amniotic fluid cells primary culture and passage culture in prenatal diagnosis

Objective: To investigate a successful amniotic fluid cells culture method,compare different methods between amniotic fluid cells primary culture and passage culture.Methods:35 of 77 amniotic fluid cells with primary culture,42 amniotic fluid cells with passage culture.Results:(1) Primary culture was more simple,we cloud short the time and get the longer chromosomes in primary culture,but got the less chromosomes.(2)We cloud get the more chromosomes to analysis in passage culture,when the number of cell clones was less then 10.Conclusions: We should choose primary culture,when we could get the more cell clones;We should choose passage culture,when we could get the less cell clones.