Metabolic Profile Changes in Mangalarga Marchador Horses Subjected to A Hypercaloric Diet Evaluated by Proton NMR Spectroscopy.

ABSTRACT The incidence of equine metabolic syndrome, a condition that results in endocrinopathic laminitis, is increasing worldwide. Although it is well known that the development of this syndrome depends on imbalances in energy metabolism and genetic traits, its pathophysiology remains unclear. Hydrogen nuclear magnetic resonance (1H NMR) is a powerful tool used to compare metabolic profiles and to discriminate metabolites in living beings. The aim was to apply this technology to detect blood biomarkers candidates in horses that were previously demonstrated to developed metabolic changes when subjected to induced obesity. Nine Mangalarga Marchador horses received a hypercaloric diet for 5 months and serum metabolomic analysis was performed before, during, and after the diet period. The 1H NMR results were subjected to multivariate analysis and NMR analysis allowed to identify six compounds (alanine, threonine, choline, α-glucose, β-glucose, and creatinine), and observe the increasing choline level over the assessment period in four animals. A hypercaloric diet altered the metabolic profile of horses, with an individual bias in the time at which these changes occurred. This study is the first to describe metabolomic compounds in Mangalarga Marchador horses subjected to a hypercaloric diet rich in non-structural carbohydrates.