Ethylene insensitive mutation improves Arabidopsis plant tolerance to NO2 exposure

Abstract Ethylene signaling was addressed, for the first time, in plant responses to nitrogen dioxide (NO2) by comparatively analyzing the performance of Arabidopsis ethylene insensitive 2 (ein2-1) with wild-type (WT) plants. Following NO2 fumigation, severe leaf wilting and chlorosis occurred in WT plants, but much less symptoms were observed in ein2-1. The activities of superoxide dismutase (SOD), peroxidase (PRX) and catalase (CAT) were 39%, 92%, and 11% higher, respectively, in ein2-1 than in WT following NO2 exposure. Although glutathione contents and the ratio of its reduced form (GSH) to oxidized form (GSSG) were decreased by NO2, an obviously alleviated degree was detected in ein2-1 relative to WT. Correspondingly, the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), and electrolyte leakage were 25%, 24%, and 29% lower, respectively, in ein2-1 than in WT. The difference of oxidative stress between two tested genotypes was also revealed by the leaf staining regarding the production and distribution of H2O2, superoxide anion (O2˙ˉ), and cell death. The genes involved in antioxidation or oxidation-reduction processes mostly presented a stronger expression in ein2-1 than in WT under NO2 stress. The photosynthesis-related parameters including chlorophyll and soluble sugar contents, net photosynthetic rate (Pn), and ribulose bisphosphate carboxylase/oxygenase (Rubisco) activity and gene expression, and chlorophyll fluorescence parameters were affected, generally, to a lesser degree in ein2-1 than in WT following NO2 fumigation. The enzymatic activities and gene expressions of invertases mostly displayed a higher level in ein2-1 relative to WT following NO2 fumigation. For example, the activities of cytoplasmic, cell wall and vacuolar invertases were 76%, 26%, and 26% higher, respectively, in ein2-1 than in WT. Together, these data suggest that ethylene signal insensitivity efficiently improves plant tolerance to NO2 exposure, and the possible mechanisms might be correlated with leaf antioxidative defense, photosynthesis-related processes, and sucrose metabolisms.