In vitro Propagation of Walnut （Juglans regia） by Nodal Explants

Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA + NAA or BA + Kin. BA at concentration (2 mg L BA) gave the best results in percentage response and number of shoots/plant (100%, 2.84) respectively. While, Kin at 3 mg L gave the highest average shoot length (2.81 cm). However, MS medium supplemented with 2.5 mg L + 0.5 mg L Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants (3.7, 8.2) respectively. While the highest length of shoot (4.7 cm) in MS medium supplemented with 3 mg L Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg LIBA (Indole-3-butyric acid) was obtained .