Effect of Medium, Growth Regulators and Activated Charcoal on Plant Regeneration from in vitro Culture of Dioscorea japonica TUNBERG

This study was carried out to investigate the optimal propagation condition through stem node tissue culture of yam supplemented with concentration levels of growth regulators and activated charcoal in 1 /8MS white media. For shoot induction and plant regeneration from yam stem node tissue wre more effectivein combination of auxin, kinetin and activated charcoal than combination of auxin and kinetin. Shoot induction and plant regeneration were more effective in 1/8 medium suplemented with 1AA and activated charcoal than in white medium, but in case of white medium, Shoot induction and plant regeneration wre effective in culture medium supplemented with NAAa and activated charcoal . Growth status in optimal propagation condition from yam stem node tissue brought the results of multiple plant, elongated plant height and increase in number of leaves and roots, but tissue culture effect by kinds of media in number of multiple plant were more effective in 1 /8MS medium than in white medium.