Effects of humic acids, para-aminobenzoic acid and ascorbic acid on the N-nitrosation of the carbamate insecticide propoxur and on the mutagenicity of nitrosopropoxur

Abstract Nitrosation of the carbamate insecticide propoxur at pH 3 and 37°C was determined colorimetrically and found to be time- and sodium nitrite concentration-dependent. Nitrosated propoxur was mutagenic when exposed to the seeds of the higher plant Arabidopsis thaliana but the formation of nitrosopropoxur, the presumed mutagen, was inhibited by humic acids, para -aminobenzoic acid and ascorbic acid. These agents also reduced the mutagenicity of performed nitrosopropoxur.