Ricerche Sul Meristema Apicale della Radice. VII L'attività di Alcuni Enzimi Della Glicolisi, del Ciclo di Krebs e di Quello dei Pentoso Fosfati

Abstract Studies on root apical meristem. VII. Activities of some enzymes of glycolysis, of the Krebs cycle and of the pentose phosphate cycle. — The enzymes assayed are: aldolase, glyceraldheyde-3-phosphate: NAD oxidoreductase, triosephosphate isomerase, fumarate hydratase, aconitate hydratase, glucose-6-phosphate: NADP oxidoreductase, 6-phosphogluconate: NADP oxidoreductase. The determinations were made on pea roots (Pisum sativum cv. Alaska) which were obtained by germinating seeds on moist vermiculite, in the dark, at 25° C, either for 2 or 7 days. In both cases samples were collected from the meristematic zone (0-2 mm from the apex) and from the extending zone (2-4 mm from the apex). All the enzymes determined are present both in the meristematic and in the extending zone of the root. If referred to a fresh weight basis, their activities are always greater in the meristem; if referred to a dry weight or to a protein basis, the values in the meristematic zone are nearly equal to, or higher than those ...