Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment

A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa. The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized. Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times. By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified. Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment. More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites. On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500). These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.