[Association of IL-10 promoter polymorphism and plasma levels with susceptibility to vocal cords leukoplakia].

Objective To investigated the association between interleukin-10（IL-10）-1082/-819/-592 promoter polymorphism, plasma IL-10 levels and risk of vocal leukoplakia. Methods A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed. Genotypes for the IL-10 gene （ IL-10 -1082 G/A, -819 C/T and -592 C/A） were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA. Results Smoking and alcohol consumption increased the risk of vocal leukoplakia （OR = 4.73, 95% CI：2.4 - 9.1; OR = 5.70, 95% CI：2.9 - 11.2, P < 0.01）. Patients with vocal cord leukoplakia had significantly higher frequencies of AC at -592 and -819 （OR = 1.93,95% CI： 0.97-3.81,P=0.05） and AG at -1082 （OR = 2.14 , 95%CI：0.87-5.27, P=0.092） than control. Vocal leukoplakiapatients had higher concentration of plasma IL-10 （21.6 ± 0.5）pg/ml（x±s） than controls （19.0±1.1） pg/ml（t = 2.08, P = 0.043） and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations （24.3±5.7）pg/ml compared with the ATA haplotype （19.9±4.7）pg/ml（t = -2.64, P = 0.008）. Conclusions IL-10 -1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia, and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism. Key words: Vocal cords; Leukoplakia, oral; Interleukin-10; Polymorphism, single