Reconstructing an ovary cancer microenvironment for in vitro 3D drug testing: A new avenue for ovary cancer research.

OBJECTIVE To elaborate on in vitro 3D ovary cancer models that can mimic the in vivo condition of an ovary cancer microenvironment for anticancer drug discovery. Methods Literature research was conducted in NCBI, ScienceDirect, and Pubchem databases. A total of 19 articles relevant to the search terms were included in this review. RESULTS The ovary cancer niche has been described as the home of cancer stem cells, multipotent stromal cells, fibroblasts, blood and lymphatic vessels, growth factors, extracellular matrix protein (ECM). Cell cancer behaviour toward anticancer drugs can also be altered by deposition of ECM. A human ovary decellularized protocol generates ovary scaffold. Incorporation of cancer stem cells (CSC) in the ovary cancer niche thus yield 3D reconstructed microenvironment for further anticancer drug testing. CONCLUSIONS The 3D reconstructed microenvironment provides a platform for further anticancer drug testing.