Classification of cytochrome P450s in common bean ( Phaseolus vulgaris L.)

Genome-wide analysis of Phaseolus vulgaris revealed the presence of 247 cytochrome P450 (CYP450) genes belonging to 10 clans consisting of 47 families. Of the 247 genes, 15 of them are pseudogenes. CYP71 clan was found to represent all A-type genes (144) belonging to 19 families. Nine clans possessed 28 families containing 103 non-A-type genes. Majority (112) of the CYP genes of P. vulgaris were found to contain single intron. Out of 582 introns, 357 exhibited phase zero. P. vulgaris A-type CYPs (141) showed the presence of signature F141G132XG141R137 R140XC141P133G131. While the signature sequence F93XXG93XR85XC93XG92 was found in 93 non-A-type CYPs. However, genes belonging to CYP74 family possessed a unique signature starting with W. CYP genes of P. vulgaris classified in the present investigation can be used as reference for annotating and classifying the CYPs from other legumes.