Comparative Genomic and Physiological Analyses of a Superoxide Dismutase Mimetic (SODm-123) for Its Ability to Respond to Oxidative Stress in Tomato Plants.

Superoxide dismutases (SODs) are a group of enzymes that have a crucial role in controlling oxidative stress in plants. Here, we synthesized an environmentally friendly SOD mimic, SODm-123, from L-aspartic acid and manganese oxide. SODm-123 showed similar enzymatic activity to Mn-SOD. To gain insights into the role of SODm-123 in oxidative stress tolerance, a series of experiments were conducted to assess the physiological and molecular responses of tomato plants when treated with SODm-123. The results showed that the levels of O2-• and H2O2 in tomato cells were affected by SODm-123 treatment, indicating that SODm-123 can control oxidative stress like Mn-SOD. The results also exhibited that SODm-123 increased the contents of photosynthetic pigments. However, it was noted that SODm-123 resulted in a reduction in the content of soluble sugar and MDA. These results indicate that SODm-123 promoted the efficiency of photosynthesis by regulating the content of H2O2. To further investigate the role of SODm-123 in controlling oxidative stress, a transcriptome analysis was used to identify differentially expressed genes (DEGs) associated with SODm-123 treatment. The results indicated that SODm-123 treatment resulted in 341 differentially expressed genes (DEGs) in treated tomato leaves at 96 h after treatment. Kyoto encyclopedia of genes and genomes (KEGG) revealed that DEGs were involved in pathways such as photosynthetic pigment biosynthesis, ABC transporters, sugar metabolism, and MAPK signaling, which further confirmed a positive role of SODm-123 in improving stress tolerance in plants. Overall, the results of this study suggest that SODm-123 promotes the growth and development of tomato seedlings and therefore can be used as a potential growth-promoting agent for plants.